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exponential plateau model  (GraphPad Software Inc)


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    GraphPad Software Inc exponential plateau model
    Quantitative analyses of the Tat activity in the TatA Q8 variants.A, plot of the transport kinetics of the TatA Q8 variants in pulse-chase experiments. Identities of wtTatA and TatA Q8 variants are shown in the right. Mature/total denotes the ratio of the mature band to the sum of the precursor and mature bands revealed by autoradiography of the samples from the pulse-chase experiment transporting SufI; chase time is denoted on the abscissa. Lines show best fits of an <t>exponential</t> model to the points arising from at least two biological replicates for each mutant. B, plot of the initial velocities (V0) in the TatA Q8 variants transporting SufI. Exact values of the V0 are shown above the bars. TatA Q8 variants are grouped based on classification of the amino acids.
    Exponential Plateau Model, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/exponential+plateau+model/exponential+plateau+function/pmc10124905-575-23-48
    Average 90 stars, based on 1 article reviews
    exponential plateau model - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "The polar amino acid in the TatA transmembrane helix is not strictly necessary for protein function"

    Article Title: The polar amino acid in the TatA transmembrane helix is not strictly necessary for protein function

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2023.102998

    Quantitative analyses of the Tat activity in the TatA Q8 variants.A, plot of the transport kinetics of the TatA Q8 variants in pulse-chase experiments. Identities of wtTatA and TatA Q8 variants are shown in the right. Mature/total denotes the ratio of the mature band to the sum of the precursor and mature bands revealed by autoradiography of the samples from the pulse-chase experiment transporting SufI; chase time is denoted on the abscissa. Lines show best fits of an exponential model to the points arising from at least two biological replicates for each mutant. B, plot of the initial velocities (V0) in the TatA Q8 variants transporting SufI. Exact values of the V0 are shown above the bars. TatA Q8 variants are grouped based on classification of the amino acids.
    Figure Legend Snippet: Quantitative analyses of the Tat activity in the TatA Q8 variants.A, plot of the transport kinetics of the TatA Q8 variants in pulse-chase experiments. Identities of wtTatA and TatA Q8 variants are shown in the right. Mature/total denotes the ratio of the mature band to the sum of the precursor and mature bands revealed by autoradiography of the samples from the pulse-chase experiment transporting SufI; chase time is denoted on the abscissa. Lines show best fits of an exponential model to the points arising from at least two biological replicates for each mutant. B, plot of the initial velocities (V0) in the TatA Q8 variants transporting SufI. Exact values of the V0 are shown above the bars. TatA Q8 variants are grouped based on classification of the amino acids.

    Techniques Used: Activity Assay, Pulse Chase, Autoradiography, Mutagenesis

    Related Articles

    Pulse Chase:

    Article Title: The polar amino acid in the TatA transmembrane helix is not strictly necessary for protein function
    Article Snippet: .. Mature-to-total (sum of precursor and mature) data from the pulse-chase experiment were plotted against each time point, which were then fitted with the exponential plateau model deriving from the first-order reaction model, y = ( y max − y 0 ) × e − k t , using GraphPad Prism. y max was defined as the maximum of the mature-to-total value, and y 0 represents the initial mature-to-total value at the end of the pulse. t represents time in minutes. ..

    Article Title: Functional analysis of the polar amino acid in TatA transmembrane helix
    Article Snippet: .. Mature-to-total (sum of precursor and mature) data from the pulse-chase experiment was plotted against each time point, which were then fitted with the exponential plateau model deriving from the first-order reaction model, y = ( y max − y 0 ) × e − kt , using GraphPad Prism. y max was defined as the maximum of the mature-to-total value, and y 0 represents the initial mature-to-total value at the end of the pulse. t represents time in minutes. the initial velocity ( V 0 ), representing the transport rate when y = 0, was obtained by V 0 = y max × k , with the units of the reciprocal minutes. ..

    Software:

    Article Title: Association of Monogenic vs Polygenic Hypercholesterolemia With Risk of Atherosclerotic Cardiovascular Disease
    Article Snippet: .. The percent variance in LDL-C explained versus number of SNVs in the model were assessed using an exponential plateau model with Prism 8 software (GraphPad Software, Inc.) ..



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    GraphPad Software Inc exponential plateau model
    Quantitative analyses of the Tat activity in the TatA Q8 variants.A, plot of the transport kinetics of the TatA Q8 variants in pulse-chase experiments. Identities of wtTatA and TatA Q8 variants are shown in the right. Mature/total denotes the ratio of the mature band to the sum of the precursor and mature bands revealed by autoradiography of the samples from the pulse-chase experiment transporting SufI; chase time is denoted on the abscissa. Lines show best fits of an <t>exponential</t> model to the points arising from at least two biological replicates for each mutant. B, plot of the initial velocities (V0) in the TatA Q8 variants transporting SufI. Exact values of the V0 are shown above the bars. TatA Q8 variants are grouped based on classification of the amino acids.
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    Quantitative analyses of the Tat activity in the TatA Q8 variants.A, plot of the transport kinetics of the TatA Q8 variants in pulse-chase experiments. Identities of wtTatA and TatA Q8 variants are shown in the right. Mature/total denotes the ratio of the mature band to the sum of the precursor and mature bands revealed by autoradiography of the samples from the pulse-chase experiment transporting SufI; chase time is denoted on the abscissa. Lines show best fits of an <t>exponential</t> model to the points arising from at least two biological replicates for each mutant. B, plot of the initial velocities (V0) in the TatA Q8 variants transporting SufI. Exact values of the V0 are shown above the bars. TatA Q8 variants are grouped based on classification of the amino acids.
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    Image Search Results


    Quantitative analyses of the Tat activity in the TatA Q8 variants.A, plot of the transport kinetics of the TatA Q8 variants in pulse-chase experiments. Identities of wtTatA and TatA Q8 variants are shown in the right. Mature/total denotes the ratio of the mature band to the sum of the precursor and mature bands revealed by autoradiography of the samples from the pulse-chase experiment transporting SufI; chase time is denoted on the abscissa. Lines show best fits of an exponential model to the points arising from at least two biological replicates for each mutant. B, plot of the initial velocities (V0) in the TatA Q8 variants transporting SufI. Exact values of the V0 are shown above the bars. TatA Q8 variants are grouped based on classification of the amino acids.

    Journal: The Journal of Biological Chemistry

    Article Title: The polar amino acid in the TatA transmembrane helix is not strictly necessary for protein function

    doi: 10.1016/j.jbc.2023.102998

    Figure Lengend Snippet: Quantitative analyses of the Tat activity in the TatA Q8 variants.A, plot of the transport kinetics of the TatA Q8 variants in pulse-chase experiments. Identities of wtTatA and TatA Q8 variants are shown in the right. Mature/total denotes the ratio of the mature band to the sum of the precursor and mature bands revealed by autoradiography of the samples from the pulse-chase experiment transporting SufI; chase time is denoted on the abscissa. Lines show best fits of an exponential model to the points arising from at least two biological replicates for each mutant. B, plot of the initial velocities (V0) in the TatA Q8 variants transporting SufI. Exact values of the V0 are shown above the bars. TatA Q8 variants are grouped based on classification of the amino acids.

    Article Snippet: Mature-to-total (sum of precursor and mature) data from the pulse-chase experiment were plotted against each time point, which were then fitted with the exponential plateau model deriving from the first-order reaction model, y = ( y max − y 0 ) × e − k t , using GraphPad Prism. y max was defined as the maximum of the mature-to-total value, and y 0 represents the initial mature-to-total value at the end of the pulse. t represents time in minutes.

    Techniques: Activity Assay, Pulse Chase, Autoradiography, Mutagenesis